目的 应用高效液相色谱-电喷雾离子阱质谱法(HPLC-ESI/MSn)研究金匮肾气丸总苷的有效部位在肝匀浆及血中的代谢差异情况。方法 通过大鼠肝匀浆体外代谢及应用HPLC-ESI/MSn联用技术,比较总苷的体内外代谢差异及相关性。结果 根据负离子模式下的分子离子峰获得化合物相对分子质量信息,总苷在大鼠肝匀浆温孵20 min共检测到13个化学成分。与总苷在大鼠体内血浆中代谢成分相比较,除肝匀浆特有15号峰五没食子酰葡萄糖外,两者含有8个相同的原型成分。结论 血浆中共检测到了9个代谢成分,而肝脏中仅发现了4个代谢成分,且都与血浆中代谢成分所共有,表明血浆中这4个代谢产物,为总苷中原型成分经肝脏代谢产生,该研究结果对揭示总苷化学成分在体内外的代谢机制具有重要意义。
Abstract
OBJECTIVE To study the metabolic differences of the effective fractions of total glycosides of Jinkui Shenqi Pills in liver homogenate and blood by high performance liquid chromatography-electrospray ion trap mass spectrometry (HPLC-ESI/MSn). METHODS The in vitro metabolism of the glycosides was studied using rat liver homogenate and the in vivo metabolism was investigated in rats. The metabolites were detected by HPLC-ESI/MS/MS. According to the molecular ion peak in negative ion mode, the molecular weight information of the compound was obtained. RESULTS A total of 13 chemical components were detected in the rat liver homogenate after incubation for 20 min. Eight prototype components were detected in both liver homogenate and plasma while pentagalloylglucose was only found in the liver homogenate. A total of nine metabolites were detected in plasma, and only four metabolites were found in the liver, which were shared by those in plasma. CONCLUSION The four common metabolites are produced through liver metabolism of the total glycosides. The RESULTS of this study have important implications for revealing the metabolic mechanisms of the total glycoside constituents in vitro and in vivo.
关键词
金匮肾气丸总苷 /
有效部位 /
肝匀浆 /
代谢
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Key words
Jinkui Shenqi Pills total glycoside /
effective part /
liver homogenate /
metabolism
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中图分类号：
R284
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参考文献
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脚注
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基金
哈尔滨商业大学校级科研项目资助(18XN083)
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